Gallery

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Sparse deconvolution assisted microscopy

SD-SIM


Original Image
Modified Image

System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Label:
  Lamp1-mCherry;
  MitoTracker® Deep Red FM;
  Hoechst H1399;
  Tubulin-EGFP.
Description:
Left: raw SD-SIM; Right: Sparse SD-SIM.

Link to the paper


System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Label:
  Tom20-mCherry;


Link to the paper

Expansion 4.5-fold


Original Image
Modified Image

System:
Expansion microscopy 4.5-fold.
Objective:
Wide-field objective (100×/1.45 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  Sec61β-GFP.
Description:
Left: raw expansion; Right: Sparse expansion.

Link to the paper System paper

Original Image
Modified Image

System:
Expansion microscopy 4.5-fold.
Objective:
Wide-field objective (100×/1.45 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  α-tubulin immunostaining.
Description:
Left: raw expansion; Right: Sparse expansion.

Link to the paper System paper

2D-SIM


Original Image
Modified Image

System:
2D-SIM.
Objective:
TIRF objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  LAMP1-EGFP.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.

Link to the paper System paper

Original Image
Modified Image

System:
2D-SIM.
Objective:
TIRF objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  LipidSpot 488.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.

Link to the paper System paper

Original Image
Modified Image

System:
2D-SIM.
Objective:
TIRF objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  LysoView 488.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.

Link to the paper System paper

TIRF-SIM


Original Image
Modified Image

System:
High NA TIRF-SIM.
Objective:
TIRF objective (100×/1.7 HI oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
60-80-100-120 nm DNA origami.
Label:
  Cy5, two sites fluorescently labeled.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.

Link to the paper System paper

Gated STED


Original Image
Modified Image

System:
Gated STED microscope.
Objective:
Wide-field objective (100×/1.40 oil, HCX PL APO, Leica)
Cell line:
HeLa cell.
Label:
  anti-Tom20;
  anti-Tubulin;
Description:
Left: raw STED; Right: Sparse STED.

Link to the paper

Original Image
Modified Image

System:
Gated STED microscope.
Objective:
Wide-field objective (100×/1.40 oil, HCX PL APO, Leica)
Cell line:
HeLa cell.
Label:
  anti-Mab414;
Description:
Left: raw STED; Right: Sparse STED.

Link to the paper

MTPM


Original Image
Modified Image

System:
MTPM.
Objective:
Micro-objective with NA 0.7
Sample:
Brain of atransgenic mouse (in vivo).
Label:
  Thy1-GFP
Description:
Left: raw MTPM; Right: Sparse MTPM.
Color-coded 3D volume (50~160 μm).

Link to the paper System paper

SPoSI

Original Image
Modified Image

System:
Label-free super-resolution microscopy (SPoSI).
Objective:
Wide-field objective (NA 0.9)
Sample:
Gold nanorod (20 nm x 300 nm).
Description:
Left: raw wide-field (552 nm laser); Right: SPoSI.

Link to the paper

Light-field endoscope

Original Image
Modified Image

System:
Light-field endoscope.
Objective:
GRIN lens (NA 0.5).
Sample:
USAF target and live worm.
Description:
Left: 3D live worm; Right: tilted USAF target.

Link to the paper

SACD

Original Image
Modified Image

System:
Spinning disk confocal.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  Qdots 525.
Description:
Left: confocal; Right: SACD.
Huge FOV 3D volume (10μm in depth);
20 frame per SACD reconstruction.

Link to the paper

DL-SIM

Original Image
Modified Image

System:
2D-SIM.
Objective:
Wide-field objective (100×/1.45 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  MitoTracker Green.
Description:
Left: wide-field; Right: DL-SIM.

Link to the paper

Original Image
Modified Image


System:
High NA TIRF-SIM.
Objective:
TIRF objective (100×/1.7 HI oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
INS-1 cell.
Label:
  LifeAct-EGFP.
Description:
Left: TIRF; Right: DL-SIM.
Long-term 1h imaging, 3400 frames.

Link to the paper


ODT

ODT

System:
ODT.
Objective:
Wide-field objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Description:
The process of cell division in hours.
0~10 μm and the plane at 7 μm is visualized on the left.
From Liangyi Chen.

Link to the paper

SIM assisted ODT

Single channel

Original Image
Modified Image

Merged

Original Image


System:
2D-SIM assisted ODT.
Objective:
Wide-field objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
  MitoTracker Green.
Description:
Left panel: ODT (left) at 5μm plane (0~6μm in total); 2D-SIM (right).
Right panel: 2D-SIM (red) + ODT (gray).
From Liangyi Chen.


Miniaturized light-field microscopy

System concept

Original Image

System on a live-mouse

Original Image


System: Miniaturized light-field microscopy.
Objective: GRIN lens (0.5 NA)
Camera: CMOS camera
Sample: Live-mouse.
Label: GCaMP6s in vivo.
Description: From Changliang Guo.

Link to the paper