Gallery
SN2N
SD-SIM
System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Description:
RL-SN2N on SD-SIM.
Link to the paper
System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Description:
3D RL-SN2N on SD-SIM.
Link to the paper
System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Description:
3D RL-SN2N on SD-SIM.
Link to the paper
System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Description:
3D RL-SN2N on SD-SIM.
Link to the paper
System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Description:
RL-SN2N on SD-SIM.
Link to the paper
STED
System:
STED.
Objective:
Wide-field objective (100×/1.49 oil, Olympus) Cell line:
COS-7 cell.
Description:
SN2N on STED.
Link to the paper
GI-SIM
2D-SIM
System:
2D-SIM.
Objective:
Wide-field objective (100×/1.49 oil, Olympus) Cell line:
COS-7 cell.
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Description:
SN2N on SIM.
Link to the paper
Sparse deconvolution-assisted microscopy
SD-SIM
System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Label:
Lamp1-mCherry;
MitoTracker® Deep Red FM;
Hoechst H1399;
Tubulin-EGFP.
Description:
Left: raw SD-SIM; Right: Sparse SD-SIM.
Link to the paper
System:
SD-SIM.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (C14440-20UP, Hamamatsu, Japan).
Cell line:
COS-7 cell.
Label:
Tom20-mCherry;
Link to the paper
Expansion 4.5-fold
System:
Expansion microscopy 4.5-fold.
Objective:
Wide-field objective (100×/1.45 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
Sec61β-GFP.
Description:
Left: raw expansion; Right: Sparse expansion.
Link to the paper System paper
System:
Expansion microscopy 4.5-fold.
Objective:
Wide-field objective (100×/1.45 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
α-tubulin immunostaining.
Description:
Left: raw expansion; Right: Sparse expansion.
Link to the paper System paper
2D-SIM
System:
2D-SIM.
Objective:
TIRF objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
LAMP1-EGFP.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.
Link to the paper System paper
System:
2D-SIM.
Objective:
TIRF objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
LipidSpot 488.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.
Link to the paper System paper
System:
2D-SIM.
Objective:
TIRF objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
LysoView 488.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.
Link to the paper System paper
TIRF-SIM
System:
High NA TIRF-SIM.
Objective:
TIRF objective (100×/1.7 HI oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
60-80-100-120 nm DNA origami.
Label:
Cy5, two sites fluorescently labeled.
Description:
Left: raw 2D-SIM; Right: Sparse 2D-SIM.
Link to the paper System paper
Gated STED
System:
Gated STED microscope.
Objective:
Wide-field objective (100×/1.40 oil, HCX PL APO, Leica)
Cell line:
HeLa cell.
Label:
anti-Tom20;
anti-Tubulin;
Description:
Left: raw STED; Right: Sparse STED.
Link to the paper
System:
Gated STED microscope.
Objective:
Wide-field objective (100×/1.40 oil, HCX PL APO, Leica)
Cell line:
HeLa cell.
Label:
anti-Mab414;
Description:
Left: raw STED; Right: Sparse STED.
Link to the paper
MTPM
System:
MTPM.
Objective:
Micro-objective with NA 0.7
Sample:
Brain of atransgenic mouse (in vivo).
Label:
Thy1-GFP
Description:
Left: raw MTPM; Right: Sparse MTPM.
Color-coded 3D volume (50~160 μm).
Link to the paper System paper
SPoSI
System:
Label-free super-resolution microscopy (SPoSI).
Objective:
Wide-field objective (NA 0.9)
Sample:
Gold nanorod (20 nm x 300 nm).
Description:
Left: raw wide-field (552 nm laser); Right: SPoSI.
Link to the paper
Endoscope
System:
Volumetric endoscope probe.
Objective:
GRIN lens (NA 0.5).
Sample:
USAF target and live worm.
Description:
Left: 3D live worm; Right: tilted USAF target.
Link to the paper
Smart palm-size optofluidic hematology analyzer
System:
A smart Palm-size Optofluidic Hematology Analyzer based on a miniature fluorescence microscope and a microfluidic platform to lighten the device to improve its portability.
Objective:
GRIN lens (NA 0.5).
Sample:
Leukocyte.
Description:
Tracking of the leukocytes.
Link to the paper
SACD
System:
Spinning disk confocal.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
Qdots 525.
Description:
Left: confocal; Right: SACD.
Huge FOV 3D volume (10μm in depth);
20 frame per SACD reconstruction.
Link to the pre-print
System:
Spinning disk confocal.
Objective:
Wide-field objective (100×/1.3 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
Qdots 525.
20 frame per SACD reconstruction.
Link to the pre-print
PANEL
STORM
Objective:
TIRF objective (100×/1.45 oil, Nikon)
Camera:
EMCCD camera (iXon Ultra 897, Andor)
Cell line:
COS-7 cell.
Label:
α-tubulin labeled with Alexa Fluor 647.
Description:
Left: STORM; Right: rFRC resolution map.
Link to the paper
System:
STORM
Objective:
TIRF objective (100×/1.45 oil, Nikon)
Camera:
EMCCD camera (iXon Ultra 897, Andor)
Cell line:
COS-7 cell.
Label:
α-tubulin labeled with Alexa Fluor 647.
Description:
Left: STORM; Right: rFRC resolution map.
Link to the paper
DL-SIM
System:
2D-SIM.
Objective:
Wide-field objective (100×/1.45 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
MitoTracker Green.
Description:
Left: wide-field; Right: DL-SIM.
Link to the paper
System:
High NA TIRF-SIM.
Objective:
TIRF objective (100×/1.7 HI oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
INS-1 cell.
Label:
LifeAct-EGFP.
Description:
Left: TIRF; Right: DL-SIM.
Long-term 1h imaging, 3400 frames.
Link to the paper
ODT
System:
ODT.
Objective:
Wide-field objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Description:
The process of cell division in hours.
0~10 μm and the plane at 7 μm is visualized on the left.
From Prof. Liangyi Chen.
Link to the paper
SIM-assisted ODT
Single channel
Merged
System:
2D-SIM assisted ODT.
Objective:
Wide-field objective (100×/1.49 oil, Olympus)
Camera:
sCMOS camera (Flash 4.0 V3, Hamamatsu, Japan)
Cell line:
COS-7 cell.
Label:
MitoTracker Green.
Description:
Left panel: ODT (left) at 5μm plane (0~6μm in total); 2D-SIM (right).
Right panel: 2D-SIM (red) + ODT (gray).
From Prof. Liangyi Chen.
Miniaturized light-field microscopy
System concept
System on a live-mouse
System: Miniaturized light-field microscopy.
Objective: GRIN lens (0.5 NA)
Camera: CMOS camera
Sample: Live-mouse.
Label: GCaMP6s in vivo.
Description: From Changliang Guo.